Fluorescein CAS# 2321-07-5

Fluorescein is a synthetic organic compound available as a dark orange/red powder slightly soluble in water and alcohol. It is widely used as a fluorescent tracer for many applications.
Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein has an absorption maximum at 494 nm and emission maximum of 521 nm (in water). The major derivatives are fluorescein isothiocyanate (FITC) and, in oligonucleotide synthesis, 6-FAM phosphoramidite.

Fluorescein CAS# 2321-07-5

Fluorescein Basic information
Product Name: Fluorescein
Synonyms: Fluorescein, C.I. 45350, synthesis grade;Fluorescein, C.I. 45350, extra pure;FluoresceinC.I.45350;Fluorescein, laser grade, pure, 99%;Fluorescein, pure;Fluorescein,highpuritystandard;D&CYellow#7;Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-dihydroxy-
CAS: 2321-07-5
MF: C20H12O5
MW: 332.31
EINECS: 219-031-8
Product Categories: Fluorescent;Xanthene;FUNDUSCEIN;Aromatics;Fluorescent Labels & Indicators;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;E-FFluorescent Probes, Labels, Particles and Stains;Fluorescein and Derivatives;Fluorescent Labels;Stains and Dyes;Stains&Dyes, A to
Mol File: 2321-07-5.mol
Fluorescein Structure
Fluorescein Chemical Properties
Melting point 320 °C(lit.)
Boiling point 429.44°C (rough estimate)
density 1.2739 (rough estimate)
refractive index 1.5000 (estimate)
storage temp. room temp
solubility Solubility Insoluble in water, ether, benzene, chloroform; soluble in ethanol, methanol, acetone, Iethyl acetate, N,N-dimethylformamide
Colour Index 45350
form Powder
pka 2.2, 4.4, 6.7(at 25℃)
color Red to orange
PH Range Pink uorescence (4.0) to green uorescence (6.0)
Water Solubility insoluble
λmax 493.5nm, 496nm, 460nm, 515nm
Merck 14,4159
BRN 94324
Stability: Stable. Combustible. Incompatible with strong oxidizing agents.
Major Application Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer
Biological Applications Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity
LogP 2.980 (est)
CAS DataBase Reference 2321-07-5(CAS DataBase Reference)
EPA Substance Registry System Fluorescein (2321-07-5)

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