Fluorescein is a synthetic organic compound that appears as a dark orange/red powder and is slightly soluble in water and alcohol. It is widely used as a fluorescent tracer for many applications.
Fluorescein is a fluorescent group commonly used in microscopes, dye lasers as gain media, forensic and serological detection of potential blood stains, and dye tracing. The maximum absorption wavelength of fluorescein is 494 nm, and the maximum emission wavelength is 521 nm (in water). The main derivatives are fluorescein isothiocyanate (FITC) and 6-FAM phosphorous amide in oligonucleotide synthesis.
Fluorescein also has an equal absorbance point at 460 nm (all pH values absorb the same). Fluorescein is also known as a color additive (D&C Yellow No. 7). The disodium salt form of fluorescein is called uranium or D&C yellow.
The color of its aqueous solution changes from green to orange depending on the observation method: through reflection or transmission, as observed in a bubble level, where fluorescein is added as a colorant to the alcohol filled with alcohol. Tube to increase the visibility of bubbles and the accuracy of the instrument. A more concentrated solution of fluorescein may even appear red.
Fluorescein Uses
- Fluorescein is an intermediate of merbromine red, which can be used as a fluorescence analysis reagent to determine chlorine, bromine, iodine, and sulfur ions; Oxidation-reduction indicator reagent; Biological staining agents.
- Fluorescein (Resorcinol phthalein) is a synthetic organic photosensitive dye compound. Fluorescein is a fluorescent tracer that can be applied to medicine, biology, and tissues infected with tumors.
- A fluorescent tracer used for various applications. It is used as a tool in ophthalmology to diagnose corneal abrasions, corneal ulcers, and herpetic corneal infections. It can attach to certain biologically active molecules, allowing biologists to target fluorescent groups to specific proteins or structures within cells.
- Fluorescein is used as a fluorescent tracer in many applications, including as a gain medium in dye lasers, detecting potential blood stains in forensic and serology, and in dye tracing. It is used to locate multiple muscular ventricular septal defects and confirm the presence of any residual defects during open heart surgery. Apply it to the teeth to expose dental plaque.